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README.md
README.md

Scripts for the Publication:

Introgression patterns between house mouse subspecies and species reveal genomic windows of frequent exchange

Ullrich KK, Linnenbrink M, Tautz D

Data sources

Genome mapping files for Mus musculus domesticus GER, Mus musculus domesticus FRA, Mus musculus domesticus IRA, Mus musculus musculus AFG, Mus musculus castaneus CAS and Mus spretus SPRE were obtained from http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/m_m_domesticus/genomes_bam/, http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/m_m_musculus/genomes_bam/, http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/m_m_castaneus/genomes_bam/, http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/m_spretus/genomes_bam/.

For mapping details please look into the original publication (Harr et al. 2016) http://www.nature.com/article-assets/npg/sdata/2016/sdata201675/extref/sdata201675-s7.docx.

Get masking regions for individual samples and natural populations

For masking genomic regions in natural populations which showed low coverage based on the genomic mapping BAM files we only considered the stable chromosomes (chr1-chr19,chrX,chrY,chrM) from the reference GRCm38 mm10 http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/mouse/.

The BAM files were processed with 'genomeCoverageBed' from the bedtools software suite (Quinlan and Hall 2010) to obtain site specific genome coverage and further united with 'unionBedGraphs'.

Population specific masking

The per population combined coverage was further processed to only retain regions with a coverage smaller than 5 resulting as the masking regions.

genomeCoverageBed example for the population Mus musculus musculus AFG:

#example for populaton Mmm_AFG:
#
#used BAM files:
#
#AFG1_396.bam
#AFG2_413.bam
#AFG3_416.bam
#AFG4_424.bam
#AFG5_435.bam
#AFG6_444.bam

REFERENCE=mm10.fasta

for file in *.bam; do genomeCoverageBed -ibam $file -bga -g $REFFERENCE > $file".bga";done

unionBedGraphs example for the population Mus musculus musculus AFG:

INPUT1=AFG1_396.bam.bga
INPUT2=AFG2_413.bam.bga
INPUT3=AFG3_416.bam.bga
INPUT4=AFG4_424.bam.bga
INPUT5=AFG5_435.bam.bga
INPUT6=AFG6_444.bam.bga

OUTPUT=Mmm_AFG.combined.bga

unionBedGraphs -i $INPUT1 $INPUT2 $INPUT3 $INPUT4 $INPUT5 $INPUT6 | awk -v OFS='\t' 'BEGIN {sum=0} {for (i=4; i<=NF; i++) sum+=$i; print $1,$2,$3,sum; sum=0}' > $OUTPUT

get masking region example for the population Mus musculus musculus AFG:

INPUT=Mmm_AFG.combined.bga
OUTPUT=Mmm_AFG.combined.bga.stcov5

awk '{if($4<5) print $0}' $INPUT > $INPUT".stcov5"
bedtools merge -i $INPUT".stcov5" > $INPUT".stcov5.merge"
awk -v OFS='\t' '{print $1,$2,$3,4}' $INPUT".stcov5.merge" > $OUTPUT

United masked files generated can be obtained from:

http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/introgression/masking/

Individual specific masking

For individuals regions with a coverage smaller than 5 were used as masking regions.

get masking region example for individual 396 of the population Mus musculus musculus AFG:

INPUT=AFG1_396.bam.bga
OUTPUT=AFG1_396.bam.bga.stcov5

awk '{if($4<5) print $0}' $INPUT > $INPUT".stcov5"
bedtools merge -i $INPUT".stcov5" > $INPUT".stcov5.merge"
awk -v OFS='\t' '{print $1,$2,$3,4}' $INPUT".stcov5.merge" > $OUTPUT

used software:

SNP and INDEL calling

For SNP and INDEL calling the BAM files were processed with 'samtools mpileup' and 'bcftools call' (Li et al. 2011) with relaxed quality options to retain information in CNV regions. Each chromosome was analyzed seperately.

samtools mpileup | bcftools call example for chromosome 1 for the population Mus musculus musculus AFG:

#example for population Mmm_AFG:
#
#used BAM files:
#
#AFG1_396.bam
#AFG2_413.bam
#AFG3_416.bam
#AFG4_424.bam
#AFG5_435.bam
#AFG6_444.bam

REFERENCE=mm10.fasta

PLOIDY=ploidy.txt
#chrX    1       171031299       M       1
#chrY    1       91744698        M       1
#chrY    1       91744698        F       0
#chrM    1       16299   F       1
#chrM    1       16299   M       1
#*       *       *       M       2
#*       *       *       F       2

SAMPLES=AFG_samples.txt
#396     M
#413     M
#416     M
#424     M
#435     F
#444     M

OUTPUT=mpileup.q0Q10.chr1.Mmm_AFG.bcfcall.mv.vcf

INPUT1=AFG1_396.bam
INPUT2=AFG2_413.bam
INPUT3=AFG3_416.bam
INPUT4=AFG4_424.bam
INPUT5=AFG5_435.bam
INPUT6=AFG6_444.bam

BAMLIST=Mmm_AFG.list

echo $INPUT1 >> $BAMLIST
echo $INPUT2 >> $BAMLIST
echo $INPUT3 >> $BAMLIST
echo $INPUT4 >> $BAMLIST
echo $INPUT5 >> $BAMLIST
echo $INPUT6 >> $BAMLIST

#example for chromosome 1

samtools mpileup -q 0 -Q 10 -A -d 99999 -t DP,AD,ADF,ADR -r chr1 -uf $REFERENCE -b $BAMLIST | bcftools call -O v -f GQ -m -v --ploidy-file $PLOIDY -S $SAMPLES > $OUTPUT
bgzip $OUTPUT
tabix $OUTPUT".gz"

used software:

  • samtools 1.3.1 (using htslib 1.3.1)
  • bcftools 1.3.1 (using htslib 1.3.1)
  • bgzip v1.3
  • tabix v1.3

All mpileup generated for each population can be obtained from:

http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/introgression/mpileup_pop_mv/vcf/mpileup/

NOTE: For each population all analyzed chromosomes were merged into one file.

K80 distance calculation

Get population specific SNPs

To get population specific CONSENSUS VCF files the VCF file produced with 'bcftools call' was first re-coded into population specific VCF files with 'vcftools' (Danecek et al. 2011). Further the population specific VCF file containing multiple individuals was parsed with 'vcfparser.py mvcf2consensus' to obtain a CONSENSUS VCF file for each population. This CONSENSUS VCF files were used to generate pseudo-genomes files per natural population with 'vcfparser.py vcf2fasta' using also the masking regions (see "Get masking regions for individual samples and natural populations").

vcftools example for chromosome 1 for the population Mus musculus musculus AFG:

#example for population Mmm_AFG:
#
#VCF IDs:
#
#396
#413
#416
#424
#435
#444

POPIDS=AFG.vcf.ids

echo "396" >> $POPIDS
echo "413" >> $POPIDS 
echo "416" >> $POPIDS
echo "424" >> $POPIDS
echo "435" >> $POPIDS
echo "444" >> $POPIDS

GZVCF=mpileup.q0Q10.chr1.Mmm_AFG.bcfcall.mv.vcf.gz
OUTPUT=Mmm_AFG.mpileup.q0Q10.chr1.bcfcall.mv.remIndels

vcftools --gzvcf $GZVCF --remove-indels --recode --recode-INFO-all --non-ref-ac-any 1 --keep $POPIDS --out $OUTPUT

All re-coded vcf files can be obtained from:

http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/introgression/mpileup_pop_mv/vcf/recode/

NOTE: For each population all analyzed chromosomes were merged into one file.

vcfparser.py mvcf2consensus example for the population Mus musculus musculus AFG:

#example for population Mmm_AFG:
#
#VCF IDs:
#
#396
#413
#416
#424
#435
#444

INPUT=Mmm_AFG.mpileup.q0Q10.chr1.bcfcall.mv.remIndels.recode.vcf
OUTPUT=Mmm_AFG.mpileup.q0Q10.chr1.bcfcall.mv.remIndels.recode.consensus

python vcfparser.py mvcf2consensus -ivcf $INPUT -o $OUTPUT -cdp 11 -chr chr1 -samples 396,413,416,424,435,444 -id Mmm_AFG.mv

All CONSENSUS vcf files can be obtained from:

http://wwwuser.gwdg.de/~evolbio/evolgen/wildmouse/introgression/mpileup_pop_mv/vcf/consensus/

NOTE: For each population all analyzed chromosomes were merged into one file.

vcfparser.py vcf2fasta example for the population Mus musculus musculus AFG:

#example for population Mmm_AFG:
#

REFERENCE=mm10.fasta
INPUT=Mmm_AFG.mpileup.q0Q10.chr1.bcfcall.mv.remIndels.recode.consensus.vcf
OUTPUT=Mmm_AFG.mpileup.q0Q10.chr1.bcfcall.mv.remIndels.recode.consensus.chr1
MASKFILE=Mmm_AFG.combined.bga.stcov5

python vcfparser.py vcf2fasta -ivcf $INPUT -o $OUTPUT -R $REFERENCE -samples Mmm_AFG.mv -chr chr1 -ibga $MASKFILE -cov2N 4

All pseudo-genome FASTA files can be obtained from:

used software:

Calculate dK80 distance between populations using the CONSENSUS pseudo-genome files

Simulation

To simulate genomes, first we estimated the number of pair-wise segregating sites with the CONSENSUS pseudo-genome files adding the reference mm10. Subsequently we used